Current Research Projects
Metabolic labeling of complex microbial communities (MSCA-DN METAMIC3)
Start: since 01.10.2025
Funding: HORIZON TMA MSCA Doctoral Networks
METAMIC3 - Metaproteome-based leveraged microbiome management in the context of One Health
People
This project aims to applying protein-labeling techniques such as protein-stable isotope probing (SIP) and click chemistry approaches such as bioorthogonal non-canonical amino acid tagging (BONCAT) for an enhanced identification of metabolic active microbes (WP3). We will apply these techniques to reconstruct metabolic networks in microbial communities originating from various environmental and organismal samples, identify the cellular response of single species, or simplify MOCK communities relevant to specific objectives described in WP1. This will reveal the influence of antimicrobial effectors in different microbiomes. In addition, the replication of phages triggered by microbe-phage interactions or environmental stress will be confirmed by BONCAT (in collaboration with AUAS).
How Segatella copri deals with oxygen: Mechanisms for the conversion of O2 and peroxides in an obligate anaerobic gut bacterium
Start: since 01.01.2026
Funding: DFG SPP 2474 Illuminating gene functions in the human gut microbiome
People
Methabreed - Reduction of methane emissions in dairy cows
Sustainable reduction of methane emissions in dairy farms considering breeding strategies, improved feed efficiency and animal health (project 4)
Duration: 2025-2027
Funding by Bundesanstalt für Landwirtschaft und Ernährung
Partners
- Förderverein Bioökonomieforschung e.V. (FBF)
- Vereinigte Informationssysteme Tierhaltung w.V. (vit)
- Justus-Liebig-Universität Gießen (JLU)
- Christian-Albrechts-Universität zu Kiel (CAU)
Correlation of metal homeostasis and catabolism of Prevotellaceae in the rumen
Start: since 01.01.2025
Funding: DFG (327953272)
People
Publications from previous project:
Deusch S, Bok E, Schleicher L, Seifert J, Steuber J (2019) Occurrence and function of the Na+-translocating NADH:Quinone oxidoreductase in Prevotella spp. Microorganisms 7(5), 117, doi: 10.3390/microorganisms7050117
Schleicher L, Fritz G, Seifert J, Steuber J (2020) Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na+-translocating NADH:quinone oxidoreductase from oxidative damage. Archive of Microbiology 202, 1263–1266, doi: 10.1007/s00203-019-01805-x
Trautmann A, Schleicher L, Deusch S, Gätgens J, Steuber J, Seifert J (2020) Short-chain fatty acids modulate metabolic pathways and membrane lipids in Prevotella bryantii B14. Proteomes. 8, 28; doi:10.3390/proteomes8040028
Schleicher L, Trautmann A, Stegmann D, Fritz G, Gätgens J, Bott M, Hein S, Simon J, Seifert J, Steuber J (2021) A sodium-translocating module linking succinate production to formation of a membrane potential in Prevotella bryantii. Applied and Environmental Microbiology. doi.10.1128/AEM.01211-21.
Trautmann A, Schleicher L, Pfirrmann J, Boldt C, Steuber J, Seifert J (2021) Na+-coupled respiration and reshaping of extracellular polysaccharide layer counteract monensin-induced cation permeability in Prevotella bryantii B14. International Journal of Molecular Sciences 22: 10202; doi.10.3390/ijms221910202.
Trautmann A, Schleicher L, Koch A, Günther J, Steuber J, Seifert J. (2022) A shift towards succinate-producing Prevotella in the ruminal microbiome challenged with monensin. Proteomics. e2200121. doi: 10.1002/pmic.202200121
Hau JL, Schleicher L, Herdan S, Simon J, Seifert J, Fritz G, Steuber J (2023) Functionality of the Na+-translocating NADH:quinone oxidoreductase and quinol:fumarate reductase from Prevotella bryantii inferred from homology modeling. Archives in Microbiology. 206(1):32. doi: 10.1007/s00203-023-03769-5.
Host-Microbiome Interaction: Implications for cellular and global energy metabolism of dairy cows (WiMiQ)
WiMiQ
2024- 2026
DFG: Projektnummer 202989534
In collaboration with FLI Braunschweig (Dr J. Frahm, Prof. S. Dänicke), Uni Bonn (Prof. H. Sauerwein)
Publications
from previous project MitoCow
Tröscher-Mußotter J, Saenz JS, Grindler S, Meyer J, Kononov SU, Mezger B, Borda-Molina D, Frahm J, Dänicke S, Camarinha-Silva A, Huber K, Seifert J (2021) Microbiome clusters disclose physiologic variances in dairy cows challenged by calving and lipopolysaccharides.
mSystems 6(5):e00856; doi.org/10.1128/mSystems.00856-21
Tröscher-Mußotter J, Deusch S, Borda-Molina D, Frahm J, Dänicke S, Camarinha-Silva A, Huber K, Seifert J (2022) Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges.
Frontiers in Microbiology 13. doi: 10.3389/fmicb.2022.1000750
Project in the DFG Research Unit ‘Appearance and physiological relevance of inositol phosphates and myo-inositol in the domesticated fowl’ FOR 2601
Project time: 2022-2025
People
Groups
- Functional Microbiology of Livestock
- Microbial Ecology of Livestock
- Institute of Animal Science
Publications
Naithani H, Rios-Galicia B, Camarinha Silva A, Seifert J. (2024) Strategies to enhance cultivation of anaerobic bacteria from gastrointestinal tract of chicken. J. Vis. Exp. 10:207, e66570, doi: 10.3791/66570.
2023-2026
Funding: Bundesanstalt für Landwirtschaft und Ernährung
Groups
This is a collaborative project with Prof. Amélia Camarinha-Silva und Prof. Markus Rodehutscord.
Partnership program The Hebrew University of Jerusalem – University of Hohenheim
2024-2025
Dr. Kfir Sharabi (Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem)
Terminated Research Projects
Duration: 2017-2022
DFG (SE 2059/3-1)
People
- Dr. Andrej Trautmann
- Prof. Dr. Julia Fritz-Steuber
- Prof. Dr. Jana Seifert
Publications
Deusch S, Bok E, Schleicher L, Seifert J, Steuber J (2019) Occurrence and function of the Na+-translocating NADH:Quinone oxidoreductase in Prevotella spp. Microorganisms 7(5), 117, doi: 10.3390/microorganisms7050117
Schleicher L, Fritz G, Seifert J, Steuber J (2020) Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na+-translocating NADH:quinone oxidoreductase from oxidative damage. Archive of Microbiology 202, 1263–1266, doi: 10.1007/s00203-019-01805-x
Trautmann A, Schleicher L, Deusch S, Gätgens J, Steuber J, Seifert J (2020) Short-chain fatty acids modulate metabolic pathways and membrane lipids in Prevotella bryantii B14. Proteomes. 8, 28; doi:10.3390/proteomes8040028
Schleicher L, Trautmann A, Stegmann D, Fritz G, Gätgens J, Bott M, Hein S, Simon J, Seifert J, Steuber J (2021) A sodium-translocating module linking succinate production to formation of a membrane potential in Prevotella bryantii. Applied and Environmental Microbiology. doi.10.1128/AEM.01211-21.
Trautmann A, Schleicher L, Pfirrmann J, Boldt C, Steuber J, Seifert J (2021) Na+-coupled respiration and reshaping of extracellular polysaccharide layer counteract monensin-induced cation permeability in Prevotella bryantii B14. International Journal of Molecular Sciences 22: 10202; doi.10.3390/ijms221910202.
Trautmann A, Schleicher L, Koch A, Günther J, Steuber J, Seifert J. (2022) A shift towards succinate-producing Prevotella in the ruminal microbiome challenged with monensin. Proteomics. e2200121. doi: 10.1002/pmic.202200121
Hau JL, Schleicher L, Herdan S, Simon J, Seifert J, Fritz G, Steuber J (2023) Functionality of the Na+-translocating NADH:quinone oxidoreductase and quinol:fumarate reductase from Prevotella bryantii inferred from homology modeling. Archives in Microbiology. 206(1):32. doi: 10.1007/s00203-023-03769-5.
01.07.2017 - 2022
People
- M.Sc. Johanna Tröscher-Mußotter
- Prof. Dr. Korinna Huber
- Prof. Dr. Jana Seifert
- Prof. Dr. rer. nat. Amélia Camarinha Silva
Groups
- Fg. Funktionelle Mikrobiologie be Nutztieren
- Fg. Funktionelle Anatomie der Nutztiere
- Fg. Mikrobielle Ökologie bei Nutztieren
- Hohenheim Center for Livestock Microbiome Research (HoLMiR)
- Institut für Nutztierwissenschaften
Publications
Tröscher-Mußotter J, Saenz JS, Grindler S, Meyer J, Kononov SU, Mezger B, Borda-Molina D, Frahm J, Dänicke S, Camarinha-Silva A, Huber K, Seifert J (2021) Microbiome clusters disclose physiologic variances in dairy cows challenged by calving and lipopolysaccharides.
mSystems 6(5):e00856; doi.org/10.1128/mSystems.00856-21
Tröscher-Mußotter J, Deusch S, Borda-Molina D, Frahm J, Dänicke S, Camarinha-Silva A, Huber K, Seifert J (2022) Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges.
Frontiers in Microbiology 13. doi: 10.3389/fmicb.2022.1000750
Project within DFG Research Unit ‘Appearance and physiological relevance of inositol phosphates and myo-inositol in the domesticated fowl’ FOR 2601
Projektbeginn: 01.05.2018
Projektende: 31.12.2021
People
Publications
Röhm K, Gonzalez-Uarquin F, Harmel RK, Trung MN, Diener M, Fiedler D, Huber K, Seifert J. (2021) Investigation of a potential electrogenic transport-system for myo-inositol in the small intestine of laying hens. British Poultry Science; doi.org/10.1080/00071668.2021.1958301
Röhm K, Diener M, Huber K, Seifert J. (2021) Characterization of the cecal smooth muscle contraction in laying hens. Veterinary Sciences 8(6), 91; doi.org/10.3390/vetsci8060091
Start: 01.08.2019
Wilhelm Schaumann Stiftung
People
- M.Sc. Alina Kurz
- Prof. Jana Seifert
Collaborators
This is a collaborative project together with Prof. Amélia Camarinha-Silva, Prof. Markus Rodehutscord and Prof. Jörn Bennewitz.
Publications
Kurz A, Seifert J (2021) Factors Influencing Proteolysis and Protein Utilization in the Intestine of Pigs: A Review.
Animals 11(12):3551; doi.org/10.3390/ani11123551
Sarpong N, Seifert J, Bennewitz J, Rodehutscord M, Camarinha-Silva A (2024) Microbial signatures and enterotype clusters in fattening pigs: implications for nitrogen utilization efficiency.
Frontiers in Microbiology.15. doi: 10.3389/fmicb.2024.1354537
Schmid M, Weishaar R, Seifert J, Camarinha-Silva A, Rodehutscord M, Bennewitz J (2024) Genomic analyses of nitrogen utilization efficiency, its indicator trait blood urea nitrogen and the relationship to classical growth performance and feed efficiency traits in a Landrace x Piétrain crossbred population.
Journal of Animal Breeding and Genetics. doi: 10.1111/jbg.12864
Project in the DFG Research Unit ‘Appearance and physiological relevance of inositol phosphates and myo-inositol in the domesticated fowl’ FOR 2601
Project time: 2018-2022
People
Groups
- Functional Microbiology of Livestock
- Microbial Ecology of Livestock
- Institute of Animal Sciences
Publications
Roth C, Sims T, Rodehutscord M, Seifert J, Camarinha-Silva A. (2022) The active core microbiota of two high-yielding laying hen breeds fed with different levels of calcium and phosphorus.
Frontiers in Physiology 13. doi:10.3389/fphys.2022.951350.
Project time: 2019-2022
Funding: EU, BMBF
Researchers
Groups
- Fg. Funktionelle Mikrobiologie bei Nutztieren
- Institut für Nutztierwissenschaften
Publications
Serrano-Villar S, Tincati C, Raju SC, Sáenz JS, Moreno E, Bargiela R, Cabello A, Sendagorta E, Kurz A, Perez Molina JA, de Benito A, Hov JR, Fernandez-Lopez L, Muriel A, del Campo R, Moreno S, Trøseid M, Seifert J, Ferrer M. (2023) Microbiome-derived cobalamin and succinyl-CoA as biomarkers for improved screening of anal cancer.
Nature Medicine. 29. 1738-1749. doi:10.1038/s41591-023-02407-3
Baden-Württemberg, MWFK, Junior-Professuren Programm, 2014 – 2017